Anti-ZP4 Antibody

ZP4 antibody

Boster Bio Anti-ZP4 Antibody (Catalog # A07440). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A07440
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-ZP4 Antibody

View all ZP4 Antibodies

SKU/Catalog Number

A07440

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ZP4 Antibody (Catalog # A07440). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ZP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07440)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ZP4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07440 is reactive to ZP4 in Human

Applications

A07440 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

59.4kDa

Background of ZP4

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.

Harris J.D., DNA Seq. 4:361-393(1994).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For ZP4 (Source: Uniprot.org, NCBI)

Gene Name

ZP4

Full Name

Zona pellucida sperm-binding protein 4

Weight

59.4kDa

Superfamily

ZP domain family

Alternative Names

zinc finger and homeodomain protein 1; zinc fingers and homeobox 1; zinc fingers and homeoboxes 1; zinc fingers and homeoboxes protein 1; zinc-fingers and homeoboxes 1 ZP4 ZBP, ZP1, ZPB, Zp-4 zona pellucida glycoprotein 4 zona pellucida sperm-binding protein 4|zona pellucida protein B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ZP4, check out the ZP4 Infographic

ZP4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ZP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-ZP4 Antibody

Question

Can you help my question with product A07440, anti-ZP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Carter

Verified customer

Asked: 2019-10-22

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07440 anti-ZP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-22

Question

I see that the anti-ZP4 antibody A07440 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-16

Question

I was wanting to use your anti-ZP4 antibody for WB for human oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human oocyte identification?

R. Collins

Verified customer

Asked: 2018-12-13

Answer

You can see on the product datasheet, A07440 anti-ZP4 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-13

Question

We are currently using anti-ZP4 antibody A07440 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

K. Anderson

Verified customer

Asked: 2013-07-25

Answer

The anti-ZP4 antibody (A07440) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-07-25

Order DetailsPrice
A07440-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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